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Whole blood is very unstable due to proteolytic degradation. The result is that blood samples older than 36 hours are no longer viable for flow cytometric examination. For the same reason unstabilised whole blood is unsuitable for use as an EQA sample. TransFix® was developed by scientists at UK NEQAS as a solution to this problem. When added to whole human blood it stabilises the profile and inhibits proteolytic deterioration.