The Bio-Nobile Paramagnetic Particle based technology Products
BN P & S offer a number of solutions for the processing of proteins and peptides: For general purification DEAE, CM, antiGST and antibody particles.
The QuickPick™ SML (Small-Medium-Large - scalable for various sample sizes) purification kits provide a fast and simple means of purifying proteins from a variety of samples. The technique does not require any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation.
For Mass spectroscopy and Proteomics we offer one approach for those worried about the loss of material due to the pre-processing: optimize your strategy using using TheGmax data mining package
Available from Bio-Nobile are methods used in the fractionation of proteins, based on weak ion exchange. They are designed to help in the evaluation of the protein fractionation prior to actual purification. This Technical Note describes methods for weak cation exchange using carboxymethyl particles (CM), and weak anion exchange using diethylaminoethyl (DEAE) particles.
See the full list of available application notes from Bio-Nobile
Download Technical Note as PDF file
Products for proteomics and protein purification
Demonstration of the fractionation properties of CM
BN P & S also offers advanced protein purification and analysis services. The proteins can be native or recombinant and the samples can be for example bacteria or mammalian cells or mammalian tissue material. Apart from magnetic particle based methods, BN P & S also offers other methods:
1. Sample preparation using extraction and precipitation methods (e.g. using chaothropic salt or organic solvent extractions, ammonium sulphate precipitations)
2. Capture and Chromatography purifications, using Ion Exchange, Gel Filtration, HIC and/or Affinity
3. Buffer changes, concentrations
4. Freeze drying
Read more about our choices in the Services section.
Ready to use Protein ladders from GeneDireX, stable at room temperature.
The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
The new BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa.